vector name paav expression class addgene no Search Results


91
Addgene inc htert rpe1 cells
Htert Rpe1 Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PSICOR Inc pgcsamenzsg1
Pgcsamenzsg1, supplied by PSICOR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pgg pip greengate vectors
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Pgg Pip Greengate Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pet flag tev lic cloning vector
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Pet Flag Tev Lic Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc mammalian expression vector pcmv p16 ink4a
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Mammalian Expression Vector Pcmv P16 Ink4a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc binary vectors pdirect 22c
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Binary Vectors Pdirect 22c, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc ppd95 79 vector
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Ppd95 79 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc cas9 expression vector px458 pspcas9 bb 2agfp
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Cas9 Expression Vector Px458 Pspcas9 Bb 2agfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc l3135 vector
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
L3135 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pbcag mrfp
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Pbcag Mrfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc pcagpbase
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Pcagpbase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc plvx ires puro vector
The basic <t>GreenGate</t> expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Plvx Ires Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The basic GreenGate expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.

Journal: Journal of Experimental Botany

Article Title: Resolving spatially distinct phytohormone response zones in Arabidopsis thaliana roots colonized by Fusarium oxysporum

doi: 10.1093/jxb/erae516

Figure Lengend Snippet: The basic GreenGate expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.

Article Snippet: The 75 pGG-PIP GreenGate vectors have been deposited at AddGene (Deposit-ID: 82532, Catalog-#: 196739-196813)

Techniques: Expressing, Clone Assay, Plasmid Preparation