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Image Search Results
Journal: Journal of Experimental Botany
Article Title: Resolving spatially distinct phytohormone response zones in Arabidopsis thaliana roots colonized by Fusarium oxysporum
doi: 10.1093/jxb/erae516
Figure Lengend Snippet: The basic GreenGate expression clone assembly principle. The different genetic modules are cloned into specific entry/donor vectors (here, A–F) defined by their 4 bp overhangs. Gene promoters (red) are typically cloned into the A-module vector and are defined by the orange and brown 4 bp overhangs in this illustration. An N-terminal tag would be cloned into the B-module vector, and is defined by the brown and navy blue overhangs. In the following assembly reaction, the promoter module and the N-tag module would self-assemble due to the matching orange 4 bp overhangs. Similarly, the other fragments donated by the C, D, E, and F module vectors also self-assemble into one long DNA fragment, which then self-assembles via the two terminal overhangs (orange from A and dark green from F, in this illustration) into the Z-module destination/expression vector. Thus, a single assembly reaction produces one ordered expression clone with fragments from six entry/donor vectors. Depending on the GreenGate toolkit used, more modules can be used to produce higher order vectors.
Article Snippet: The 75
Techniques: Expressing, Clone Assay, Plasmid Preparation